Author Topic: Novel Coronavirus (COVID-19) Thread  (Read 24695 times)

Offline metaL

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #495 on: August 15, 2020, 01:30:30 AM »

https://youtu.be/8zH9l-PJSj0

Some interesting covid related stuff in this Mark Dice video. He provides screenshots of sources, but some things need to be fact checked. Unfortunately i don't have time to do that, I'm about to work 12 hours in the blazing Florida sun.
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Offline Punk_FAS

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #496 on: August 15, 2020, 09:45:57 AM »
Fauci admits there's no reason we can't safely vote in-person this November.

Hrrmmm... Interesting choice of words.

Unfortunately i don't have time to do that, I'm about to work 12 hours in the blazing Florida sun.

RIP
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Offline metaL

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #497 on: August 15, 2020, 10:05:59 AM »
Seems accurate.
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Offline |iR|Focalor

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #498 on: August 15, 2020, 05:29:45 PM »
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Offline metaL

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #499 on: August 16, 2020, 04:56:54 PM »

https://youtu.be/60XHcinkLoM

I mean why not share this?

Big Ted. Lol
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Offline metaL

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #501 on: August 16, 2020, 06:03:49 PM »
Just IMAGINE telling a Mexican flying the Mexican flag that it's inappropriate..... IN THE USA!

Let alone MEXICO... THEIR FUCKING COUNTRY.

This is a supreme trivium of lunacy, gullibility, and ignorance.
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Offline haunted

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #502 on: August 17, 2020, 06:54:38 AM »
I've been quarantined. Dumb kid I've been working the most with got covid on his time off. He flew in Wednesday and has been working with me until now. Have to get tested later.
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Offline haunted

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #503 on: August 17, 2020, 07:42:33 AM »
Waiting on rapid test results. Crossing my fingers. I go on time off in 2 days and this would ruin my next fuckin month.

***update: tested negative. Still supposed to quarantine. I last had contact with the person Saturday.
« Last Edit: August 17, 2020, 08:08:54 AM by haunted »
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Offline quadz

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #504 on: August 17, 2020, 11:06:23 AM »
Waiting on rapid test results. Crossing my fingers. I go on time off in 2 days and this would ruin my next fuckin month.

***update: tested negative. Still supposed to quarantine. I last had contact with the person Saturday.

As I understand it, the rapid tests are often a version of the antibody tests. Do you know if yours was an antibody test?

My wife tested positive on two COVID-19 antibody tests a couple weeks ago (IgG and IgM tests.)

The claim was that testing positive for both would indicate she had already been infected for 1 to 3 weeks.

However, she has been entirely asymptomatic - as have I.

This has led us to research the antibody tests a bit, wondering about the possibility of false-positives.


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255746/
Quote
Diagnostic performance of seven rapid IgG/IgM antibody tests and the Euroimmun IgA/IgG ELISA in COVID-19 patients

The current gold standard for the diagnosis of COVID-19 is the detection of viral RNA in respiratory tract samples [1]. However, the sensitivity of nucleic acid amplification techniques is <100%. False negatives can occur, especially when using nasopharyngeal swabs (positivity rate estimated at 54–74%) because of difficulty in sampling; false negatives can also occur in patients with low viral loads (especially in patients who present at day 8 or later) and in mild cases [1].

Detection of antibodies has been proposed as an additional diagnostic tool which could help in the diagnosis of patients with suspected COVID-19 who have a negative PCR result, or in whom no respiratory sample for PCR was taken at the time of acute illness (e.g. due to lack of adequate resources during an outbreak). Seroconversion for SARS-CoV-2 is estimated to occur 7–14 days after the onset of symptoms, when the sensitivity of the PCR decreases [3,4]. Detection of antibodies could be useful in patients in whom a past asymptomatic, atypical or mild infection is suspected.
[...]
In the first quarter of 2020 more than 100 so called ‘rapid tests’ for the detection of IgM/IgG antibodies were marketed. There are, however, important concerns about the quality and diagnostic performance of rapid tests for SARS-CoV-2.
[...]
The sensitivities of the seven LFAs [lateral flow assays] included in our study for IgG were at least as good as the first CE-marked IgG ELISA during the first 3 weeks after the onset of symptoms, with a faster seroconversion for IgG LFAs. Seropositivity was >92% with all seven IgG LFAs 14–25 days after the onset of symptoms. The specificity for IgG was >97% for five of the seven LFAs, which can be considered very good given the challenging nature of the control samples used in our evaluation. The performance of the IgM LFAs, however, varied greatly, and the average dynamic trend to seropositivity was not shorter than that for IgG. For the LFAs, including IgM also did not improve the diagnostic performance. The low specificity of the IgA ELISA has since been confirmed by the manufacturer who now recommends not using the IgA ELISA for screening of asymptomatic persons.

Initial reports suggested that IgM antibodies against SARS-Cov-2 might appear earlier than IgGs, and that measuring both IgM and IgG would improve the diagnosis of SARS-Cov-2 infection [1,10]. To et al., however, found that more patients had earlier seroconversion for IgG than for IgM. In addition, they also found a 100% seroconversion for IgG antibodies, but not for IgM, 14 days after the onset of symptoms in 16 patients for whom serial serum samples were available [3]. Recently, Long et al. reported 100% seroconversion for IgG after 19 days [11]. Our results confirm these observations in a group of more than 80 patients and suggest that the antibody response to SARS-CoV-2 might be comparable to the response to SARS-CoV-1 where the three antibodiy classes IgA, IgG and IgM seroconverted simultaneously, or even 1 day earlier for IgG [12].

Combining the results of IgG LFAs and IgM LFAs did not improve the diagnostic performance, questioning the rationale for measuring IgM anti-SARS-CoV-2 antibodies.
[...]
The main expected use of antibody testing in the coming months is to confirm past COVID-19 in patients, to determine (herd) immunity, and in epidemiological studies [15]. Our results suggest that detection of IgG antibodies can be very useful if performed at least 18 days after onset of symptoms or, in asymptomatic persons, after the end of an outbreak. There is currently no clear evidence that measuring IgA or IgM is useful. Our results even suggest that it might be better not to measure IgM or IgA since this could result in a significant number of false-positive results without a significant gain in diagnostic performance.



https://www.prevention.com/health/a32957865/how-long-do-coronavirus-antibodies-last/

Quote
So, you’ve tested positive for COVID-19 antibodies—what now?
The CDC stresses that antibody tests “are not 100% accurate and some false positive results or false negative results may occur.” That means it’s possible to get a positive result for COVID-19 antibodies, but not actually have them.

The reason, per Dr. Adalja: There are various other coronaviruses that can infect humans, including some that cause the common cold. So if you felt sick but never had a confirmed case of COVID-19, it’s possible to get skewed results. Plus, if you’re only in the early days of an active infection, antibodies may not be picked up at all, because your immune system is still working on building up a response.



So................. :dohdohdoh:

It seems with antibody tests:

- you can get a false negative (for the purposes using antibodies for SARS-CoV-2 detection) if the antibody test is taken too soon after infection (e.g. less than 18 days) because your body hasn't started producing the IgG antibodies yet.

- you can get a false positive, if you still have antibodies from fighting off a cold (seasonal coronavirus)


So as the study indicated above, antibody tests appear more suited as an additional diagnostic tool in conjunction with PCR tests.

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Offline haunted

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #505 on: August 17, 2020, 12:00:01 PM »
Rapid antigen test, for a specific protein that produces an immune system response from covid, I guess.
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Offline quadz

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #506 on: August 17, 2020, 12:40:40 PM »
Rapid antigen test, for a specific protein that produces an immune system response from covid, I guess.



https://www.mdpi.com/2075-4418/10/4/202/pdf
Quote
In Vitro Diagnostic Assays for COVID-19: Recent Advances and Emerging Trends

A wide range of serology immunoassays (IAs) have also been developedthat complement the molecular assays for the diagnosis of COVID-19. The most prominent IAs areautomated chemiluminescent IA (CLIA), manual ELISA, and rapid lateral flow IA (LFIA), which detect the immunoglobulin M (IgM) and immunoglobulin G (IgG) produced in persons in response to SARS-CoV-2 infection.
[...]
SARS-CoV-2 is a large positive-sense single-stranded ribonucleic acid (RNA) virus that comprises of four structural proteins, i.e., nucleocapsid protein (NP) that holds the viral RNA, spike protein (SP),envelope protein (EP), and membrane protein (MP), that create the viral envelope.


This is from the original SARS-CoV research in early 2000s. Don't know to what degree it translates to SARS-CoV-2:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329003/
Quote
Nucleocapsid Protein as Early Diagnostic Marker for SARS

Our results suggest that N protein in the serum samples of SARS patients can be detected as early as day 1 after disease onset. Although the level of circulating N protein was highly variable from one person to another from day 1 to day 18, development of SARS-CoV N-IgG appears not to be affected by N protein during the acute phase of the infection, 7 days after the onset of symptoms. The positive detection rate of N protein in serum samples within the first 10 days of infection is higher than that detected by RT-PCR (8,9).




Well - it does at least sound like the "nucleocapsid protein detection" test is relevant to early detection.

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Offline |iR|Focalor

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #507 on: August 17, 2020, 02:17:31 PM »
Please wear a mask while posting.

Also, Quadz, it might be helpful if you could code the messageboard to insert one or two blank replies between every post so as to maintain proper social distancing.
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Offline metaL

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #508 on: August 17, 2020, 04:50:41 PM »
I'm so horrified of covid I don't take my boxers off before I sit on the toilet.

All of you are bigots if you don't do the same.

Disagree and you're literally trusting a turd. Have fun with that Trumptards.

:Duhard:
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Offline haunted

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Re: Novel Coronavirus (COVID-19) Thread
« Reply #509 on: August 18, 2020, 03:48:39 AM »
Company I work for is not even considering the doctor's order for me to quarantine a possibility.  :lolsign:

Back at work.
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